droplet digital polymerase chain reaction(English)
- technology that utilizes Taq polymerase in a standard PCR reaction to amplify a target DNA fragment from a complex sample using pre-validated primer or primer/probe assays. However, there are two distinct differences: 1) the partitioning of the PCR reaction into thousands of individual reaction vessels prior to amplification and 2) the acquisition of data at reaction end point. These factors offer the advantage of direct and independent quantification of DNA without standard curves giving more precise and reproducible data versus qPCR especially in the presence of sample contaminants that can partially inhibit Taq polymerase and/or primer annealing. This technology can be used for extremely low-target quantitation from variably contaminated samples where the sample dilution requirements to assure consistent and acceptable reaction efficiency, primer annealing and Cq values for qPCR would likely lead to undetectable target levels
- PCR, DCHS2
- Genetics, Physiology
- https://doi.org…598-017-02217-x
- https://doi.org…uro.2016.08.012