quantitative real-time PCR(English)

• a common and sensitive method for miRNAs analysis, but it is necessary to optimize conditions and minimize qRT-PCR inhibitors to achieve reliable results. An acceptable amplification efficiency was achieved by the optimization of the annealing temperature of the tested miRNAs and by the collection a supernatant volume corresponding to about 50% of the volume of TRIzol with triple chloroform extraction. These optimal extraction and PCR conditions were successfully applied to plasma miRNA screening to detect biomarker candidates for the diagnosis of acute myocardial infarction
• PCR, miRNA
• Biochemistry, Genetics
• https://doi.org…896-021-00710-w