External RNA Controls Consortium(English)

• in 2003, the NIST hosted a meeting to discuss the need for a universal RNA reference material, which could be used for gene expression profiling assays, and as a result the ERCC was formed. The Consortium assembled a library of 176 DNA sequences that could be transcribed into RNA to serve as controls in systems used to measure gene expression, available as a set of sequence-certified DNA plasmids, via SRM. Initially assessed with three different microarray platforms, pools were subsequently measured by RNA sequencing (RNA-Seq) with two second-generation (NGS) sequencing platforms
• RNA-Seq, NGS, NIST, RNA, SRM
• Genomics
• https://doi.org…2896-016-0281-x