PHA-L

Phaseolus vulgaris leukoagglutinin(English)

  • a plant lectin that is anterogradely transported by neurons in the central nervous system, and is selectively taken up by cells at iontophoretic injection sites and, when immunohistochemically demonstrated, labels individual neurons completely, including their dendrites, axons, and terminal boutons. When prepared for electron microscopy, the labeled terminals are densely and completely stained, allowing their synaptic relationships to be defined. These properties make it advantageous for studying the patterns of projection and the modes of termination of select groups of neurons in their target nuclei. Can be used as anterograde tracer for VTA projections to the VTA-nigral complex
  • DY, HRP, TB, PrV, WGA, VTA, RR, SNc
  • Microscopy, Neurons, Histochemistry, Synapse, Brain (dys)function
  • https://doi.org…jemt.1060150405
  • https://doi.org…2/cne.903140202
  • https://doi.org…nce.2008.02.003