sequence- and ligation-independent cloning(English)

• method that uses an exonuclease, T4 DNA polymerase, to generate single-stranded DNA overhangs in insert and vector sequences. These fragments are then assembled in vitro and transformed into E coli to generate recombinant DNA of interest
• DNA, PCR, iPCR
• Cloning, Molecular biology
• https://doi.org…1-61779-564-0_5